E2F-dependent transcription of the raf proto-oncogene during Drosophila development.
Nucleic Acids Res
; 29(8): 1808-14, 2001 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-11292854
ABSTRACT
D-raf, a Drosophila homolog of the raf proto-oncogene, has diverse functions throughout development and is transcribed in a wide range of tissues, with high levels of expression in the ovary and in association with rapid proliferation. The expression pattern resembles those of S phase genes, which are regulated by E2F transcription factors. In the 5'-flanking region of D-raf, four sequences (E2F sites 1-4) similar to the E2F recognition sequence were found, one of them (E2F site 3) being recognized efficiently by Drosophila E2F (dE2F) in vitro. Transient luciferase expression assays confirmed activation of the D-raf gene promoter by dE2F/dDP. Expression of Draf-lacZ was greatly reduced in embryos homozygous for the dE2F mutation. These results suggest that dE2F is likely to be an important regulator of D-raf transcription.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Transcripción Genética
/
Proteínas Portadoras
/
Transactivadores
/
Regulación del Desarrollo de la Expresión Génica
/
Proteínas de Ciclo Celular
/
Proteínas Proto-Oncogénicas c-raf
/
Proteínas de Drosophila
/
Drosophila melanogaster
Límite:
Animals
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2001
Tipo del documento:
Article