Functional differences between two morphologically distinct cell subpopulations within a human colorectal carcinoma cell line.
Braz J Med Biol Res
; 34(5): 653-61, 2001 May.
Article
en En
| MEDLINE
| ID: mdl-11323753
ABSTRACT
The LISP-I human colorectal adenocarcinoma cell line was isolated from a hepatic metastasis at the Ludwig Institute, São Paulo, SP, Brazil. The objective of the present study was to isolate morphologically different subpopulations within the LISP-I cell line, and characterize some of their behavioral aspects such as adhesion to and migration towards extracellular matrix components, expression of intercellular adhesion molecules and tumorigenicity in vitro. Once isolated, the subpopulations were submitted to adhesion and migration assays on laminin and fibronectin (crucial proteins to invasion and metastasis), as well as to anchorage-independent growth. Two morphologically different subpopulations were isolated LISP-A10 and LISP-E11. LISP-A10 presents a differentiated epithelial pattern, and LISP-E11 is fibroblastoid, suggesting a poorly differentiated pattern. LISP-A10 expressed the two intercellular adhesion molecules tested, carcinoembryonic antigen (CEA) and desmoglein, while LISP-E11 expressed only low amounts of CEA. On the other hand, adhesion to laminin and fibronectin as well as migration towards these extracellular matrix proteins were higher in LISP-E11, as expected from its poorly differentiated phenotype. Both subpopulations showed anchorage-independent growth on a semi-solid substrate. These results raise the possibility that the heterogeneity found in the LISP-I cell line, which might have contributed to its ability to metastasize, was due to at least two different subpopulations herein identified.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Colorrectales
/
Adenocarcinoma
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Moléculas de Adhesión Celular
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Proteínas de la Matriz Extracelular
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Proteínas de Neoplasias
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Braz J Med Biol Res
Año:
2001
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
BR
/
BRASIL
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BRASILE
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BRAZIL
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BRESIL