Drug-induced suppression of phospholipid synthesis in HeLa cells by inhibition of choline uptake.
Biochim Biophys Acta
; 388(2): 180-7, 1975 May 22.
Article
en En
| MEDLINE
| ID: mdl-1138894
ABSTRACT
2-(4-Anisidino)-4,6-bis (2-(diethylmethylammonium)ethylamino)-1,3,5-triazine diiodide was found to inhibit specifically the incorporation of [14 C]-choline into cold 5 percent trichloroacetic acid-insoluble materials in HeLa cells without affecting other vital areas of cellular metabolism. Further studies indicated that this drug did not affect any of the intracellular reactions leading to phosphatidylcholine formation; instead, the inhibition of lecithin synthesis was due primarily to the suppression of choline transport through the cytoplasmic membrane. The level of inhibition of the latter process was comparable to that observed for the inhibition of choline incorporation into cold 5 percent trichloroacetic acid-insoluble precipitates in whole cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfolípidos
/
Triazinas
/
Células HeLa
/
Colina
/
Compuestos de Amonio Cuaternario
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1975
Tipo del documento:
Article