A new extraction method for Acinetobacter species ODB-L2 rough form lipopolysaccharide from culture broth.
Microbios
; 105(412): 153-61, 2001.
Article
en En
| MEDLINE
| ID: mdl-11414500
ABSTRACT
The Gram-negative bacterium Acinetobacter species ODB-L2 produces lipopolysaccharide (LPS) in culture broth. The LPS could not be purified by conventional extraction methods using 90% phenol/water or 90% phenol/chloroform/petroleum ether mixed solvent. Extraction was achieved employing an admixture of chloroform, ethanol, and 4 M HCI solution. The LPS was purified from dissolving the crude extracts in 90% phenol and LPS sediment formed by addition of methanol. The LPS was characterized by chemical, biochemical, and physicochemical methods as rough form 3-hydroxydodecanoic acid rich LPS.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acinetobacter
/
Lipopolisacáridos
Idioma:
En
Revista:
Microbios
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM