Localization of metallothionein (MT) and expression of MT isoforms induced by cadmium in rat dental pulp.
Jpn J Pharmacol
; 86(1): 65-72, 2001 May.
Article
en En
| MEDLINE
| ID: mdl-11430474
ABSTRACT
We investigated the induction of metallothionein (MT) by cadmium (Cd) in the dental pulp of rat incisors. Time-course studies of MT mRNA expression after single Cd injection were observed by Northern-blot analysis. The isoform-specific expressions of MT mRNAs (MT-I, MT-II and MT-III) were observed using the reverse transcriptase-polymerase chain reaction (RT-PCR) method. Both MT-I and MT-II mRNA levels increased within 3 h, peaked at 3 h and then decreased. These findings demonstrated that MT-I and MT-II mRNA were rapidly induced by Cd in dental pulp. MT-III mRNA was constitutively expressed in rat dental pulp, but the expression level did not change by Cd treatment. The localization of MT protein in Cd-treated rat dental pulp was determined by immunohistochemical staining using anti-MT antibody against MT-I and MT-II. MT protein was localized in the specific cell type of odontoblasts (secretory odontoblasts and resting odontoblasts). In conclusion, it is likely that stained MT in the immunohistochemical study should be MT-I and/or MT-II. Furthermore, MT-I and/or MT-II in Cd-treated rat dental pulp was localized in odontoblasts, in which accumulation of Cd were reported. The cell-specific synthesis of MT may be associated with its metal storage and detoxification role in dental tissues.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cadmio
/
Pulpa Dental
/
Metalotioneína
Límite:
Animals
Idioma:
En
Revista:
Jpn J Pharmacol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón