Temperature-sensitive mutations in various genes of Escherichia coli K12 can be suppressed by the ssrA gene for 10Sa RNA (tmRNA).
Mol Genet Genomics
; 265(4): 615-21, 2001 Jun.
Article
en En
| MEDLINE
| ID: mdl-11459181
ABSTRACT
An Escherichia coli strain with a deletion in the ssrA gene that encodes 10Sa RNA (tmRNA) was used to screen for temperature-sensitive (ts) mutants whose ts phenotypes were suppressible by introduction of the wild-type ssrA gene. Mutants in four different genes were isolated. Ts mutants of this type were also obtained in a screen for mutations in thyA, the structural gene for thymidylate synthase. The ThyA activity in crude extracts prepared from the ts mutants was temperature-sensitive. The presence of the ssrA gene caused an increase in the total amount of the temperature-sensitive enzyme expressed, rather than suppressing the ts activity of the enzyme itself. SsrA-DD, a mutant form of 10Sa RNA, suppressed the ts phenotype of a thyA mutant, suggesting that degradation of a tagged peptide was not required for suppression of the ts phenotype. Considering the fact that ssrA-suppressible mutants could be isolated as temperature-sensitive mutants with mutations in different genes, it seems evident that trans-translation can occur on mRNA that is not lacking its stop codon.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Supresión Genética
/
ARN Bacteriano
/
Regulación Bacteriana de la Expresión Génica
/
Escherichia coli
/
Genes Bacterianos
/
Mutación
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Mol Genet Genomics
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón