Transduction by phiBB-1, a bacteriophage of Borrelia burgdorferi.
J Bacteriol
; 183(16): 4771-8, 2001 Aug.
Article
en En
| MEDLINE
| ID: mdl-11466280
ABSTRACT
We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce DNA, a kanamycin resistance cassette was inserted into a cloned fragment of phage DNA, and the resulting construct was transformed into B. burgdorferi CA-11.2A cells. The kan cassette recombined into a resident cp32 and was stably maintained. The cp32 containing the kan cassette was packaged by phiBB-1 released from this B. burgdorferi strain. phiBB-1 has been used to transduce this antibiotic resistance marker into naive CA-11.2A cells, as well as two other strains of B. burgdorferi. This is the first direct evidence of a mechanism for lateral gene transfer in B. burgdorferi.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacteriófagos
/
Resistencia a la Kanamicina
/
Grupo Borrelia Burgdorferi
Idioma:
En
Revista:
J Bacteriol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos