Global analysis of protein activities using proteome chips.
Science
; 293(5537): 2101-5, 2001 Sep 14.
Article
en En
| MEDLINE
| ID: mdl-11474067
ABSTRACT
To facilitate studies of the yeast proteome, we cloned 5800 open reading frames and overexpressed and purified their corresponding proteins. The proteins were printed onto slides at high spatial density to form a yeast proteome microarray and screened for their ability to interact with proteins and phospholipids. We identified many new calmodulin- and phospholipid-interacting proteins; a common potential binding motif was identified for many of the calmodulin-binding proteins. Thus, microarrays of an entire eukaryotic proteome can be prepared and screened for diverse biochemical activities. The microarrays can also be used to screen protein-drug interactions and to detect posttranslational modifications.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Proteínas Fúngicas
/
Proteoma
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Science
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos