Identification of Biomphalaria havanensis and Biomphalaria obstructa populations from Cuba using polymerase chain reaction and restriction fragment length polymorphism of the ribosomal RNA intergenic spacer.
Mem Inst Oswaldo Cruz
; 96(5): 661-5, 2001 Jul.
Article
en En
| MEDLINE
| ID: mdl-11500766
ABSTRACT
In Cuba, several Biomphalaria species have been reported such as B. orbignyi, B. schrammi, B. helophila, B. havanensis and B. peregrina; only the latter three are considered as potential hosts of Schistosoma mansoni. The specific identification of Biomphalaria species is based on anatomical and morphological characters of genital organs and shells. The correct identification of these snails is complicated by the high variation in these characters, similarity among species and in some cases by the small size of the snails. In this paper, we reported the classical morphological identification, the use of PCR and RFLP analysis of the internal transcribed spacer region of the ribosomal RNA genes for molecular identification of seven snail populations from different localities in Cuba. Using morphological and molecular analysis, we showed that among the studied Cuban Biomphalaria populations only B. havanensis and B. obstructa species were found.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biomphalaria
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Reacción en Cadena de la Polimerasa
/
ADN Espaciador Ribosómico
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
Caribe
/
Cuba
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Año:
2001
Tipo del documento:
Article
País de afiliación:
Brasil