Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats.
Proc Natl Acad Sci U S A
; 98(17): 9942-7, 2001 Aug 14.
Article
en En
| MEDLINE
| ID: mdl-11504951
ABSTRACT
Royal College of Surgeons rats are genetically predisposed to undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have examined the efficacy of subretinal transplantation of two independent human RPE cell lines each exhibiting genetic modifications that confer long-term stability in vitro. The two cell lines, a spontaneously derived cell line (ARPE19) and an extensively characterized genetically engineered human RPE cell line (h1RPE7), which expresses SV40 large T (tumor) antigen, were evaluated separately. Both lines result in a significant preservation of visual function as assessed by either behavioral or physiological techniques. This attenuation of visual loss correlates with photoreceptor survival and the presence of donor cells in the areas of rescued photoreceptors at 5 months postgrafting (6 months of age). These results demonstrate the potential of genetically modified human RPE cells for ultimate application in therapeutic transplantation strategies for retinal degenerative diseases caused by RPE dysfunction.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Epitelio Pigmentado Ocular
/
Degeneración Retiniana
/
Proteínas Proto-Oncogénicas
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Proteínas Tirosina Quinasas Receptoras
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Proteínas del Ojo
Límite:
Animals
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Humans
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2001
Tipo del documento:
Article
País de afiliación:
Reino Unido