Separation of peptides utilizing ultrahigh-temperature micellar electrokinetic chromatography.
Electrophoresis
; 22(12): 2398-402, 2001 Aug.
Article
en En
| MEDLINE
| ID: mdl-11519942
ABSTRACT
The use of ultrahigh column temperatures, up to 110 degrees C, in micellar electrokinetic capillary chromatography was investigated. The number of plates generated per unit time increased from 0.22 to 12.8 plates/s for separations at 15 degrees C and 110 degrees C, respectively. Ultrahigh-temperature micellar electrokinetic capillary chromatography was used for the separation of cyclic undecapeptides (cyclosporins). A minimum resolution of 1.39 was calculated for a critical peak pair at 110 degrees C, which is more than a 50% increase over resolution generated at 40 degrees C. During a run time of more than 90 min at 110 degrees C and at pH 9.3, no sample degradation or solvent boiling was observed.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Cromatografía Capilar Electrocinética Micelar
/
Ciclosporinas
Idioma:
En
Revista:
Electrophoresis
Año:
2001
Tipo del documento:
Article
País de afiliación:
Suiza