Increased expression and activation of poly(ADP-ribose) polymerase (PARP) contribute to retinal ganglion cell death following rat optic nerve transection.
Cell Death Differ
; 8(8): 801-7, 2001 Aug.
Article
en En
| MEDLINE
| ID: mdl-11526433
ABSTRACT
Excessive activation of the nuclear enzyme poly(ADP-ribose) polymerase (PARP) by free-radical damaged DNA mediates necrotic cell death in injury models of cerebral ischemia-reperfusion and excitotoxicity. We recently reported that secondary retinal ganglion cell (RGC) death following rat optic nerve (ON) transection is mainly apoptotic and can significantly but not entirely be blocked by caspase inhibition. In the present study, we demonstrate transient, RGC-specific PARP activation and increased retinal PARP expression early after ON axotomy. In addition, intravitreal injections of 3-aminobenzamide blocked PARP activation in RGCs and resulted in an increased number of surviving RGCs when compared to control animals 14 days after ON transection. These data indicate that secondary degeneration of a subset of axotomized RGCs results from a necrotic-type cell death mediated by PARP activation and increased PARP expression. Furthermore, PARP inhibition may constitute a relevant strategy for clinical treatment of traumatic brain injury.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nervio Óptico
/
Células Ganglionares de la Retina
/
Degeneración Retrógrada
/
Regulación Enzimológica de la Expresión Génica
/
Regulación hacia Arriba
/
Muerte Celular
/
Poli(ADP-Ribosa) Polimerasas
Límite:
Animals
Idioma:
En
Revista:
Cell Death Differ
Año:
2001
Tipo del documento:
Article
País de afiliación:
Alemania