Electrospray ionisation-mass spectrometry of hydroxamate siderophores.
Analyst
; 126(8): 1359-62, 2001 Aug.
Article
en En
| MEDLINE
| ID: mdl-11534605
ABSTRACT
Electrospray ionisation-mass spectrometry (ESI-MS) was applied to the detection of the iron complexes of the hydroxamate type siderophores ferrioxamine (FO), ferrichrome (FC) and iron(III) rhodotoluate (FR). Mass spectra of the three siderophores produced by ESI-MS were dominated by the protonated (M + 1)+ parent ions, except for FR at pH 4.3, which was present as the positively charged 11 complex. On collision with He ions, fragmentation proceeded largely via cleavage of C-N bonds. Flow injection analysis of the siderophores with detection by ESI-MS produced detection limits of 1.9 fmol for FO, 31.1 fmol for FC and 524 fmol for FR.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sideróforos
Idioma:
En
Revista:
Analyst
Año:
2001
Tipo del documento:
Article
País de afiliación:
Reino Unido