Prevention of scrapie pathogenesis by transgenic expression of anti-prion protein antibodies.
Science
; 294(5540): 178-82, 2001 Oct 05.
Article
en En
| MEDLINE
| ID: mdl-11546838
ABSTRACT
Variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy are initiated by extracerebral exposure to prions. Although prion transmission from extracerebral sites to the brain represents a potential target for prophylaxis, attempts at vaccination have been limited by the poor immunogenicity of prion proteins. To circumvent this, we expressed an anti-prion protein (anti-PrP) mu chain in Prnp(o/o) mice. Transgenic mice developed sustained anti-PrP titers, which were not suppressed by introduction of Prnp+ alleles. Transgene expression prevented pathogenesis of prions introduced by intraperitoneal injection in the spleen and brain. Expression of endogenous PrP (PrP(C)) in the spleen and brain was unaffected, suggesting that immunity was responsible for protection. This indicates the feasibility of immunological inhibition of prion disease in vivo.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Scrapie
/
Priones
/
Proteínas PrPSc
/
Anticuerpos
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
Science
Año:
2001
Tipo del documento:
Article
País de afiliación:
Suiza