Identification of a BIBAC clone that co-segregates with the petunia restorer of fertility (Rf) gene.
Mol Genet Genomics
; 266(2): 223-30, 2001 Oct.
Article
en En
| MEDLINE
| ID: mdl-11683263
ABSTRACT
Molecular markers closely linked to the Restorer of fertility (Rf) locus in petunia were sought by conducting a bulk segregant analysis. The co-segregation of markers and Rf was tested on a large BC1 population produced from two different parental lines carrying Rf. The recombination frequency between OP704 and ECCA/MACT, the two most distal markers utilized in the fine-scale mapping. was significantly different in populations derived from parents that carry different nuclear backgrounds. The fine mapping identified an amplified fragment length polymorphism (AFLP) marker that co-segregates with Rf. A petunia BIBAC library (four genome equivalents), with an average insert size of 70 kb, was constructed and screened with the linked marker. A contiguous map was constructed from three different BIBAC clones that hybridized to the marker. As a result, we have identified a 37.5-kb BIBAC clone that co-segregates with Rf.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genes de Plantas
/
ADN de Plantas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Mol Genet Genomics
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY