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Frozen tumor tissue microarray technology for analysis of tumor RNA, DNA, and proteins.
Schoenberg Fejzo, M; Slamon, D J.
Afiliación
  • Schoenberg Fejzo M; Division of Hematology/Oncology, Department of Medicine, University of California, Los Angeles, School of Medicine, Los Angeles, California, USA. mfejzo@mednet.ucla.edu
Am J Pathol ; 159(5): 1645-50, 2001 Nov.
Article en En | MEDLINE | ID: mdl-11696425
Tissue microarray technology is a new method used to analyze several hundred tumor samples on a single slide allowing high throughput analysis of genes and proteins on a large cohort. The original methodology involves coring tissues from paraffin-embedded tissue donor blocks and placing them into a single paraffin block. One difficulty with paraffin-embedded tissue relates to antigenic changes in proteins and mRNA degradation induced by the fixation and embedding process. We have modified this technology by using frozen tissues embedded in OCT compound as donor samples and arraying the specimens into a recipient OCT block. Tumor tissue is not fixed before embedding, and sections from the array are evaluated without fixation or postfixed according to the appropriate methodology used to analyze a specific gene at the DNA, RNA, and/or protein levels. While paraffin tissue arrays can be problematic for immunohistochemistry and for RNA in situ hybridization analyses, this method allows optimal evaluation by each technique and uniform fixation across the array panel. We show OCT arrays work well for DNA, RNA, and protein analyses, and may have significant advantages over the original technology for the assessment of some genes and proteins by improving both qualitative and quantitative results.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN de Neoplasias / ARN Neoplásico / Análisis de Secuencia por Matrices de Oligonucleótidos / Secciones por Congelación / Proteínas de Neoplasias / Neoplasias Tipo de estudio: Qualitative_research Límite: Animals / Female / Humans Idioma: En Revista: Am J Pathol Año: 2001 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN de Neoplasias / ARN Neoplásico / Análisis de Secuencia por Matrices de Oligonucleótidos / Secciones por Congelación / Proteínas de Neoplasias / Neoplasias Tipo de estudio: Qualitative_research Límite: Animals / Female / Humans Idioma: En Revista: Am J Pathol Año: 2001 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos