Detection of anti-p53 antibodies by ELISA using p53 synthetic or phage-displayed peptides.
J Immunol Methods
; 259(1-2): 65-75, 2002 Jan 01.
Article
en En
| MEDLINE
| ID: mdl-11730842
ABSTRACT
Anti-p53 antibodies have been detected in the sera of patients with various types of cancers. In this report, we describe the development of a new ELISA aimed at detecting anti-p53 antibodies using two peptides belonging to immunodominant epitopes of the p53 N-terminal region. We first tested the reactivity of the sera by an indirect ELISA using the peptides as a capture system. Then, the specificity of the reaction was confirmed by an inhibition assay. Two systems of peptide presentation, phage display and the streptavidin/biotin system, were evaluated. Using a panel of sera from cancer patients, both systems were found to be equally reliable, demonstrating that both peptide-based ELISAs can be used for the specific detection of anti-p53 antibodies. The presence of anti-p53 antibodies was associated with p53 alteration whether it be mutation or accumulation.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Biomarcadores de Tumor
/
Proteína p53 Supresora de Tumor
/
Anticuerpos Antineoplásicos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia