The induction of DNA puff BhC4-1 gene is a late response to the increase in 20-hydroxyecdysone titers in last instar dipteran larvae.
Mech Dev
; 110(1-2): 15-26, 2002 Jan.
Article
en En
| MEDLINE
| ID: mdl-11744365
ABSTRACT
The characterization of DNA puff BhC4-1 expression was extended and its response to 20-hydroxyecdysone investigated in Bradysia hygida and in transgenic Drosophila carrying the BhC4-1 gene. In both organisms the activation of BhC4-1 in salivary glands occurs at the end of the larval stage coinciding with the peak in ecdysone titers which induces metamorphosis. Injections of 20-hydroxyecdysone into mid-fourth instar larvae of B. hygida show that the induction of BhC4-1 expression, as well as amplification and puff C4 expansion, are late events induced by the hormone. This late response of BhC4-1 expression was also observed in transgenic salivary glands cultivated in the presence of 20-hydroxyecdysone. In vitro studies using transgenic Drosophila indicate that both repressor and activator factors regulate the timing of BhC4-1 expression in salivary glands.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas y Péptidos Salivales
/
Proteínas de Insectos
/
Dípteros
Límite:
Animals
Idioma:
En
Revista:
Mech Dev
Asunto de la revista:
EMBRIOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Brasil