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Formation of protoberberine-type alkaloids by the tubers of somatic embryo-derived plants of Corydalis yanhusuo.
Lee, Y L; Sagare, A P; Lee, C Y; Feng, H T; Ko, Y C; Shaw, J F; Tsay, H S.
Afiliación
  • Lee YL; FooYin Institute of Technology, Department of Biotechnology, Taliou, Kaohsiung County, Taiwan.
Planta Med ; 67(9): 839-42, 2001 Dec.
Article en En | MEDLINE | ID: mdl-11745021
The effects of 0.5 - 5 mg/l abscisic acid [ABA], 0.5 - 10 mg/l (2RS,3RS)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol [paclobutrazol] and 0.5 - 2 mg/l alpha-cyclopropyl-alpha-(4-methoxyphenyl)-5-pyrimidinemethanol [ancymidol], 0.5 - 5 mg/l gibberellic acid [GA(3)] and 15 - 100 mg/l polyethylene glycol [PEG] 4000 supplemented in half-strength Murashige and Skoog's (MS) medium on the production of the two major protoberberine-type alkaloids (D,L-tetrahydropalmatine and D-corydaline) by the tubers of somatic embryo-derived plants of Corydalis yanhusuo were examined. Somatic embryo derived plants were also maintained for 6 months on half-strength MS medium containing 0.1 mg/l GA(3) or 0.5 mg/l paclobutrazol. The alkaloid contents were determined by high performance liquid chromatography (HPLC). The analysis revealed that the contents of D,L-tetrahydropalmatine and D-corydaline in the tubers of somatic embryo-derived plants were higher than the marketed crude drug and varied with growth regulator/PEG-4000 treatment and the age of the plant.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alcaloides de Berberina / Papaveraceae Idioma: En Revista: Planta Med Año: 2001 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Alcaloides de Berberina / Papaveraceae Idioma: En Revista: Planta Med Año: 2001 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Alemania