Staurosporine treatment and serum starvation promote the cleavage of emerin in cultured mouse myoblasts: involvement of a caspase-dependent mechanism.
FEBS Lett
; 509(3): 423-9, 2001 Dec 14.
Article
en En
| MEDLINE
| ID: mdl-11749967
ABSTRACT
Emerin is a nuclear membrane-anchored protein which is absent or mutated in patients affected by Emery-Dreifuss muscular dystrophy. In this study, we induced apoptosis in cultured mouse myoblasts to evaluate emerin fate during the nuclear destabilization involved in programmed cell death. Emerin proteolysis was observed in myocytes during the apoptotic process. Myoblast apoptosis and emerin degradation were associated with chromatin compaction and detachment from the nuclear lamina, as detected by electron microscopy. In vivo specific inhibition of caspase 3 or caspase 6 activity completely abolished emerin proteolysis. These results show that the process of programmed cell death in muscle cells leads to emerin proteolysis, which appears to be related to caspase 6 activation and to cleavage of other nuclear envelope proteins, that share sequence homologies or functional features with emerin.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Timopoyetinas
/
Procesamiento Proteico-Postraduccional
/
Estaurosporina
/
Caspasas
/
Proteínas de la Membrana
/
Músculos
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
2001
Tipo del documento:
Article
País de afiliación:
Italia