Evidence for distinct membrane traffic pathways to melanosomes and lysosomes in melanocytes.
J Investig Dermatol Symp Proc
; 6(1): 19-24, 2001 Nov.
Article
en En
| MEDLINE
| ID: mdl-11764280
ABSTRACT
We report here morphologic and biochemical evidence that melanosomes are distinct from lysosomes. Immunofluorescence analysis revealed that TRP-1, a melanosomal membrane protein, did not colocalize with lysosomal membrane proteins LAMP1 and LGP85 in melan-a cells. Wortmannin treatment of melanocytes enhanced the distinct compartmentalization of these melanosomal/lysosomal membrane proteins by the swelling of the endosomal-lysosomal systems. The heavily melanized melanosomes did not have an altered shape, which suggests a lesser degree of membrane dynamics of stage IV melanosomes. Terminal lysosomes loaded with TR-dextran are also distinct from melanosomes, thus indicating that melanosomes are isolated from the endocytic pathway that is a representative route to lysosomes. Because AP-3 mutation leads to mistargeting of both melanosome and lysosome membrane proteins, we propose that there is a late sorting step for melanosomes and lysosomes in melanocytes after AP-3 sorting.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxidorreductasas
/
Melanosomas
/
Transporte de Proteínas
/
Lisosomas
/
Melanocitos
Límite:
Animals
Idioma:
En
Revista:
J Investig Dermatol Symp Proc
Asunto de la revista:
DERMATOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Japón