Increased accumulation of hybrid V(D)J joins in cells expressing truncated versus full-length RAGs.
Mol Cell
; 8(6): 1383-90, 2001 Dec.
Article
en En
| MEDLINE
| ID: mdl-11779512
ABSTRACT
RAG1 and RAG2 (RAGs) initiate V(D)J recombination by introducing breaks between two coding segments and flanking recombination signals (RSs). Nonhomologous end-joining (NHEJ) proteins then join the coding segments and join the RSs. In wild-type cells, both full-length and truncated ("core") RAGs lead to accumulation of "hybrid" V(D)J joins, in which an RS is appended to a different coding sequence. We now show that while hybrid joins do not accumulate in NHEJ-deficient cells that express full-length RAGs, they do accumulate in NHEJ-deficient cells that express the core RAGS; like those catalyzed by core RAGs in vitro, however, they are sealed on just one DNA strand. These results suggest a potential role for the non-core regions in repressing potentially harmful transposition events.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recombinación Genética
/
Reordenamiento Génico de Linfocito B
/
Eliminación de Secuencia
/
ADN Helicasas
/
Proteínas de Homeodominio
/
Antígenos Nucleares
/
Proteínas de Unión al ADN
Límite:
Animals
Idioma:
En
Revista:
Mol Cell
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos