Negative staining permits 4.0 A resolution with low-dose electron diffraction of catalase crystals.
Ultramicroscopy
; 90(1): 7-12, 2001 Nov.
Article
en En
| MEDLINE
| ID: mdl-11794631
ABSTRACT
Low-dose electron diffraction of thin single crystals of catalase that are negatively stained with the light-atom compound, dipotassium glucose-1,6-diphosphate, reveals Bragg reflections extending to 4.0A (= 0.40 nm). Under the same conditions, negative staining with the traditional heavy-metal salt, ammonium molybdate, also gives diffraction spots extending to 4.0 A. These results establish that negative staining of protein crystals preserves periodic structural information into the high-resolution range, unlike the widely accepted current belief that this methodology can give a resolution limited to only 20-25 A.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Catalasa
Idioma:
En
Revista:
Ultramicroscopy
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos