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Leukemia inhibitory factor decreases the arylamine N-acetyltransferase activity in human cumulus granulosa cells.
Chang, C C; Hsieh, Y Y; Chung, J G; Tsai, H D; Tsai, C H.
Afiliación
  • Chang CC; Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
J Assist Reprod Genet ; 18(12): 660-4, 2001 Dec.
Article en En | MEDLINE | ID: mdl-11808848
PURPOSE: To evaluate the activities of acetyl coenzyme A (AcCoA):arylamine N-acetyltransferase (NAT) of intact cumulus granulosa cells and the role of leukemia inhibitory factor (LIF) upon their NAT activities. METHODS: Thirty women accepted controlled ovarian hyperstimulation (COH) and oocyte retrievals. Human cumulus granulosa cells were obtained during oocyte retrievals. Using 2-aminofluorene (2-AF) and p-aminobenzoic acid (PABA) as substrates, NAT activity of all samples was determined by high pressure liquid chromatography. After the incubation with different time and concentrations of 2-AF, PABA, and LIF, 2-acetyl-aminofluorene (2-AAF) and N-acetyl-PABA (N-Ac-PABA) were measured. RESULTS: After incubation with 2.812, 5.625, 11.25, and 22.5 microM of 2-AF/PABA, their product concentrations (2-AAF/N-Ac-PABA) were 0.42/0.32, 0.76/0.58, 1.29/1.04, and 1.94/1.26 nmol/10(6) cells, respectively. After 6, 12, 18, and 24 h incubation with 11.25 microM of 2-AF/PABA, their product concentrations were 0.19/0.12, 0.56/0.4, 0.98/0.79, and 1.3/1.0 nmol/10(6) cells, respectively. After incubation with 0, 5, and 50 microM of LIF, the 2-AAF/N-Ac-PABA concentrations were 0.98/0.80, 0.70/0.52, and 0.49/0.30 nmol/10(6) cells, respectively. CONCLUSION: Intact human cumulus granulosa cells could acetylate arylamine carcinogen (2-AF) and noncarcinogens drug (PABA). LIF decreased the NAT activities. It provides a model for monitoring the effects of COH and LIF upon the oocytes.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arilamina N-Acetiltransferasa / Linfocinas / Interleucina-6 / Células de la Granulosa / Inhibidores de Crecimiento Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: J Assist Reprod Genet Asunto de la revista: GENETICA / MEDICINA REPRODUTIVA Año: 2001 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arilamina N-Acetiltransferasa / Linfocinas / Interleucina-6 / Células de la Granulosa / Inhibidores de Crecimiento Tipo de estudio: Prognostic_studies Límite: Female / Humans Idioma: En Revista: J Assist Reprod Genet Asunto de la revista: GENETICA / MEDICINA REPRODUTIVA Año: 2001 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Países Bajos