Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein.
Proc Natl Acad Sci U S A
; 99(3): 1158-63, 2002 Feb 05.
Article
en En
| MEDLINE
| ID: mdl-11818558
ABSTRACT
Isopentenyl diphosphate and dimethylallyl diphosphate serve as the universal precursors for the biosynthesis of terpenes. Although their biosynthesis by means of mevalonate has been studied in detail, a second biosynthetic pathway for their formation by means of 1-deoxy-D-xylulose 5-phosphate has been discovered only recently in plants and certain eubacteria. Earlier in vivo experiments with recombinant Escherichia coli strains showed that exogenous 1-deoxy-D-xylulose can be converted into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate by the consecutive action of enzymes specified by the xylB and ispCDEFG genes. This article describes the transformation of exogenous [U-(13)C(5)]1-deoxy-D-xylulose into a 51 mixture of [U-(13)C(5)]isopentenyl diphosphate and [U-(13)C(5)]dimethylallyl diphosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Operón
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Oxidorreductasas
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Terpenos
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Proteínas Bacterianas
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Proteínas de Escherichia coli
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Escherichia coli
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Ácido Mevalónico
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
2002
Tipo del documento:
Article
País de afiliación:
Alemania