Cuticle-degrading proteases from the entomopathogen Metarhizium flavoviride and their distribution in secreted and intracellular fractions.
Lett Appl Microbiol
; 34(2): 91-4, 2002.
Article
en En
| MEDLINE
| ID: mdl-11849501
ABSTRACT
AIMS:
The aim of this study was to analyse a native isolate of Metarhizium flavoviride (CG423) which is being developed as a myco-insecticide against grasshoppers in Brazil for the production of the cuticle-degrading subtilisin-like (Pr1), and trypsin-like (Pr2) proteases. METHODS ANDRESULTS:
The results show that Pr1 activity occurred only in medium supplemented with grasshopper cuticle (Schistocerca pallens). In contrast, Pr2 was detected in higher amounts on defined growth substrate than on cuticle-supplemented medium. Both activities were detected after 48 h of growth, suggesting that in S. pallens cuticle-containing medium these protease types are not co-ordinately expressed. Low levels of enzyme activity were detected when pre-grown mycelium was used to investigate the induction of Pr1 proteases.CONCLUSIONS:
The analysis of Pr1 and Pr2 distribution in both secreted and intracellular fractions revealed high percentage of extracellular activity, which may suggest the occurrence of an efficient mechanism of protein secretion by this fungus, probably related to substrate degradation which provides nutrients for fungal growth.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Endopeptidasas
/
Ascomicetos
/
Proteínas de Insectos
/
Saltamontes
Límite:
Animals
Idioma:
En
Revista:
Lett Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Brasil