Induction of dnaK through its native heat shock promoter is necessary for intramacrophagic replication of Brucella suis.
Infect Immun
; 70(3): 1631-4, 2002 Mar.
Article
en En
| MEDLINE
| ID: mdl-11854256
ABSTRACT
The heat shock protein DnaK is essential for intramacrophagic replication of Brucella suis. The replacement of the stress-inducible, native dnaK promoter of B. suis by the promoter of the constitutively expressed bla gene resulted in temperature-independent synthesis of DnaK. In contrast to a dnaK null mutant, this strain grew at 37 degrees C, with a thermal cutoff at 39 degrees C. However, the constitutive dnaK mutant, which showed high sensitivity to H(2)O(2)-mediated stress, failed to multiply in murine macrophage-like cells and was rapidly eliminated in a mouse model of infection, adding strong arguments to our hypothesis that stress-mediated and heat shock promoter-dependent induction of dnaK is a crucial event in the intracellular replication of B. suis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Brucella
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Regiones Promotoras Genéticas
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Proteínas HSP70 de Choque Térmico
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Respuesta al Choque Térmico
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Proteínas de Escherichia coli
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Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Infect Immun
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
EEUU
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ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
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USA