Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents.
J Virol Methods
; 103(1): 57-66, 2002 May.
Article
en En
| MEDLINE
| ID: mdl-11906733
ABSTRACT
Junin virus is the etiological agent of Argentine hemorrhagic fever, a serious rodent-borne disease. An enzyme-linked immunosorbent assay (ELISA) to detect Junin virus IgG antibodies in rodents was evaluated using sera from 27 Calomys musculinus and five Calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. The test performance was compared against an indirect immunofluorescence assay (IFA). The ELISA had a sensitivity and specificity of 100% and a reproducibility of 87.9% for samples with titers above the selected cut-off value. IFA had lower sensitivity (53%) with the same specificity. The ELISA results were similar, whether carried out on whole blood or serum samples, thus eliminating the need for serum separation. A high correlation (K=0.86) between ELISA and IFA results was obtained from 1011 wild sigmodontine and murine rodents collected within and outside of the Argentine hemorrhagic fever endemic area. These results indicate that Junin virus IgG ELISA is the most suitable assay for detection of Junin virus antibodies in rodent samples.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Roedores
/
Inmunoglobulina G
/
Ensayo de Inmunoadsorción Enzimática
/
Virus Junin
/
Infecciones por Arenaviridae
/
Anticuerpos Antivirales
/
Muridae
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
J Virol Methods
Año:
2002
Tipo del documento:
Article
País de afiliación:
Argentina