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Nuclear factor kappaB dependency of platelet-activating factor-induced angiogenesis.
Ko, Hyun-Mi; Seo, Kook Heon; Han, Su-Ji; Ahn, Kyu Youn; Choi, Il-Hwan; Koh, Gou Young; Lee, Hern-Ku; Ra, Myung Suk; Im, Suhn-Young.
Afiliación
  • Ko HM; Department of Biological Sciences, The Institute of Basic Sciences, Research Institute of Medical Science, Chonnam National University, Kwangju 500-757, Japan.
Cancer Res ; 62(6): 1809-14, 2002 Mar 15.
Article en En | MEDLINE | ID: mdl-11912159
This study investigated the mechanisms of platelet-activating factor (PAF)-induced angiogenesis in a mouse model of Matrigel implantation. PAF induced a dose- and time-dependent angiogenic response. Inhibitors of nuclear factor (NF) kappaB expression or action, including antisense oligonucleotides to the p65 subunit of NFkappaB (p65 antisense) and antioxidants such as alpha-tocopherol and N-acetyl-L-cysteine, significantly reduced PAF-induced angiogenesis. In human umbilical vein endothelial cells, PAF-induced mRNA expression and protein synthesis of various NFkappaB-dependent angiogenic factors, such as tumor necrosis factor-alpha, interleukin-1alpha, basic fibroblast growth factor, and vascular endothelial growth factor (VEGF). The PAF-induced expression of the above mentioned factors was inhibited by p65 antisense or antioxidants. A significant inhibition of the angiogenic effect of PAF was achieved by anti-VEGF antibodies or soluble VEGF receptors such as KDR and flt-1 but not by antibodies against tumor necrosis factor-alpha, interleukin-1alpha, or basic fibroblast growth factor. These data indicate that PAF enhances angiogenesis through inducing NFkappaB activation, which in turn promotes the production of angiogenic factors such as VEGF.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor de Activación Plaquetaria / FN-kappa B / Neovascularización Fisiológica Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Cancer Res Año: 2002 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor de Activación Plaquetaria / FN-kappa B / Neovascularización Fisiológica Tipo de estudio: Prognostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Cancer Res Año: 2002 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos