Nucleotide sequence of envelope protein of Japanese encephalitis virus SA14-14-2 adapted to vero cells.
DNA Seq
; 12(5-6): 437-42, 2001 Dec.
Article
en En
| MEDLINE
| ID: mdl-11913793
ABSTRACT
Live attenuated Japanese encephalitis (JE) virus SA(14)-14-2 produced in primary dog kidney cells (PDK) was adapted to Vero cells. In an effort to gain insight into the molecular basis of the biological characteristics of the SA14-14-2(Vero) strain, the 1500 nucleotide sequence encoding the envelope (E) gene which possesses major neutralizing epitopes was determined and compared with the sequences of two other attenuated JE virus strains, SA14-14-2(PHK) and SA14-14-2(PDK). The amino acid sequence of the C-terminal region (a.a. 280-500) was found to be identical for all three strains, while the N-terminal region (a.a. 1-279) shows sequence variation. The distribution of mutations in the N-terminal region was nearly the same among the three attenuated strains, suggesting that the N-terminal sequences might be related with virus-host cell specificity. However, it was found that Lys and Val (a.a. 138 and 176, respectively), known to be responsible for attenuation, are still conserved in SA(14)-14-2(Vero). Animal testing showed that SA(14)-14-2(Vero) has an attenuation phenotype similar to that of the parent SA(14)-14-2(PDK) strain in mice.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas del Envoltorio Viral
/
Virus de la Encefalitis Japonesa (Especie)
Límite:
Animals
Idioma:
En
Revista:
DNA Seq
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Corea del Sur