Extraction, isolation, and SDS-PAGE analysis of purified gastric mucin in a patient with Menetrier's disease.
Am J Gastroenterol
; 97(3): 752-5, 2002 Mar.
Article
en En
| MEDLINE
| ID: mdl-11922574
ABSTRACT
Menetrier's disease is a rare condition characterized by marked proliferation of gastric mucosa with variable mucus secretion and achlorhydria. Although crude mucus secretion and gastric aspirates have been evaluated in this disease for output of dry matter, hexosamine, fucose, protein content, and transforming growth factor alpha activity, we report for the first time the isolation, purification, and gel electrophoresis of mucin from crude mucus scrapings. The fragmentation pattern of mucin in Menetrier's disease demonstrated less large polymeric mucin than the control. There was also a band of approximately 55-65 kd M, on polyacrylamide gel electrophoresis similar to that found in gastric carcinoma or peptic ulcer, but absent in the control specimens.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Electroforesis en Gel de Poliacrilamida
/
Mucinas Gástricas
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Gastritis Hipertrófica
Límite:
Aged
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Humans
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Male
Idioma:
En
Revista:
Am J Gastroenterol
Año:
2002
Tipo del documento:
Article
País de afiliación:
Sudáfrica
Pais de publicación:
EEUU
/
ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
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USA