Genomewide analysis of mRNA processing in yeast using splicing-specific microarrays.
Science
; 296(5569): 907-10, 2002 May 03.
Article
en En
| MEDLINE
| ID: mdl-11988574
ABSTRACT
Introns interrupt almost every eukaryotic protein-coding gene, yet how the splicing apparatus interprets the genome during messenger RNA (mRNA) synthesis is poorly understood. We designed microarrays to distinguish spliced from unspliced RNA for each intron-containing yeast gene and measured genomewide effects on splicing caused by loss of 18 different mRNA processing factors. After accommodating changes in transcription and decay by using gene-specific indexes, functional relationships between mRNA processing factors can be identified through their common effects on spliced and unspliced RNA. Groups of genes with different dependencies on mRNA processing factors are also apparent. Quantitative polymerase chain reactions confirm the array-based finding that Prp17p and Prp18p are not dispensable for removal of introns with short branchpoint-to-3' splice site distances.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
ARN Mensajero
/
Proteínas Nucleares
/
Procesamiento Postranscripcional del ARN
/
Empalme del ARN
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Proteínas de Unión al ARN
/
Genoma Fúngico
/
Proteínas de Ciclo Celular
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Proteínas de Unión al ADN
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Science
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos