In vitro selection as a powerful tool for the applied evolution of proteins and peptides.
Curr Opin Chem Biol
; 6(3): 390-8, 2002 Jun.
Article
en En
| MEDLINE
| ID: mdl-12023121
ABSTRACT
New in vitro methods for the applied evolution of protein structure and function complement conventional cellular and phage-based methods. Strategies employing the direct physical linkage of genotype and phenotype, and the compartmental association of gene and product to select desired properties are discussed, and recent useful applications are described. Engineering of antibodies and other proteins, selection from cDNA libraries, and the creation of functional protein domains from completely random starting sequences illustrate the value of the in vitro approaches. Also discussed is an emerging new direction for in vitro display technology the self-assembly of protein arrays.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biosíntesis de Proteínas
/
Evolución Molecular
/
Biblioteca de Péptidos
Idioma:
En
Revista:
Curr Opin Chem Biol
Asunto de la revista:
BIOQUIMICA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos