Lipid analysis of the response of a sedimentary microbial community to polycyclic aromatic hydrocarbons.
Microb Ecol
; 43(2): 189-98, 2002 Mar.
Article
en En
| MEDLINE
| ID: mdl-12023726
ABSTRACT
Polycyclic aromatic hydrocarbons (PAH) are widespread environmental contaminants that can, under proper conditions, be degraded by microorganisms. The responses of a riverine sedimentary microbial community to PAH contamination were examined using an integrated biochemical assay that yielded data on PAH concentration, total microbial biomass, and microbial community structure and were interpreted using perturbation theory and the subsidy-stress gradient. Microbial mineralization of naphthalene, anthracene, fluorene, and phenanthrene was observed 24 h after their addition to all sediments sampled and ranged from 0.9 to 16.3% in ambient sediments and from 14.8 to 35.8% in contaminated sediments. Total microbial biomass, determined by phospholipid phosphate, increased in response to intermediate PAH concentration and decreased at sites with the highest PAH concentration (p < 0.05) during seven out of nine (78%) seasonal sampling periods. The two sampling periods that were not statistically different followed periods of high water and cold temperatures. Phospholipid fatty acid analysis of microbial community structure analysis indicated that increases in the relative abundance of gram-negative aerobes and heterotrophic eukaryotes were responsible, in part, for these observed increases in total microbial biomass. These findings (increased degradation rates, increased biomass at intermediate PAH concentrations, and altered community structure) indicate that a component of the microbial community responded to PAH as a usable input and are consistent with the predictions of perturbation theory and a subsidy-stress gradient.
Palabras clave
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hidrocarburos Policíclicos Aromáticos
/
Microbiología del Agua
/
Contaminantes Químicos del Agua
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Monitoreo del Ambiente
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Sedimentos Geológicos
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Lípidos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Microb Ecol
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos