ERK1/2 mediates PDGF-BB stimulated vascular smooth muscle cell proliferation and migration on laminin-5.
Biochem Biophys Res Commun
; 293(3): 1000-6, 2002 May 10.
Article
en En
| MEDLINE
| ID: mdl-12051759
ABSTRACT
During restenosis following arterial injury, vascular smooth muscle cells (VSMCs) form a neointimal layer in arteries by changing from a differentiated, contractile phenotype to a dedifferentiated, migratory, and proliferative phenotype. Several growth factors, cytokines, and extracellular matrix components released following injury have been implicated in these phenotypic changes. We have recently detected the expression of laminin-5, an ECM protein found predominantly in epithelial tissues, in the arterial vasculature. Here we report that ln-5 expression by VSMC is upregulated by platelet-derived growth factor (PDGF-BB), epidermal growth factor, basic fibroblast growth factor, and transforming growth factor-beta1. Adhesion to ln-5 specifically enhances PDGF-BB-stimulated VSMC proliferation and migration. PD98059, a specific inhibitor of the ERK1/2 members of the Mitogen Activated Protein kinase family, increases both VSMC adhesion to ln-5 and blocks PDGF-BB-stimulated VSMC migration on ln-5. These results suggest that adhesion to ln-5 mediates a PDGF-BB-stimulated VSMC response to vascular injury via an ERK1/2 signaling pathway.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factor de Crecimiento Derivado de Plaquetas
/
Moléculas de Adhesión Celular
/
Movimiento Celular
/
Proteínas Quinasas Activadas por Mitógenos
/
Músculo Liso Vascular
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos