Could induced apoptosis of human granulosa cells predict in vitro fertilization-embryo transfer outcome? A preliminary study of 25 women.
Eur J Obstet Gynecol Reprod Biol
; 103(2): 150-3, 2002 Jul 10.
Article
en En
| MEDLINE
| ID: mdl-12069738
ABSTRACT
OBJECTIVE:
The aim of this study was to investigate the relationship between induced apoptosis of human luteinized granulosa cells (GCs) and in vitro fertilization (IVF) outcome. STUDYDESIGN:
We induced apoptosis with interferon gamma and an anti-human Fas antibody in cultured GCs isolated from follicular fluids coming from 25 different women undergoing 25 consecutive IVF cycles. After examination of 1000 GCs stained by DAPI with a fluorescent microscope, we determined the percentages of apoptotic GCs. Ovarian, endometrial and IVF parameters were recorded for every woman.RESULTS:
We classified the women according to their induced GCs apoptosis percentages in two groups. Group 1 with a low percentage of apoptotic GCs (11.6+/-4.8%) had a significantly higher pregnancy rate (P<0.05) than group 2 with a high percentage of apoptotic GCs (59.5+/-14.8%). No other statistically significant differences were observed.CONCLUSION:
Resistance of human GCs to apoptosis might be implicated in IVF outcome.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fertilización In Vitro
/
Transferencia de Embrión
/
Células de la Granulosa
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Adult
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Eur J Obstet Gynecol Reprod Biol
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia