Visualization and functional analysis of RNA-dependent RNA polymerase lattices.
Science
; 296(5576): 2218-22, 2002 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-12077417
ABSTRACT
Positive-strand RNA viruses such as poliovirus replicate their genomes on intracellular membranes of their eukaryotic hosts. Electron microscopy has revealed that purified poliovirus RNA-dependent RNA polymerase forms planar and tubular oligomeric arrays. The structural integrity of these arrays correlates with cooperative RNA binding and RNA elongation and is sensitive to mutations that disrupt intermolecular contacts predicted by the polymerase structure. Membranous vesicles isolated from poliovirus-infected cells contain structures consistent with the presence of two-dimensional polymerase arrays on their surfaces during infection. Therefore, host cytoplasmic membranes may function as physical foundations for two-dimensional polymerase arrays, conferring the advantages of surface catalysis to viral RNA replication.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Polimerasa Dependiente del ARN
/
ARN Viral
/
Poliovirus
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Science
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos