An enzymatic fluorometric assay for pyridoxal with high specificity and sensitivity.
Biosci Biotechnol Biochem
; 66(5): 1152-4, 2002 May.
Article
en En
| MEDLINE
| ID: mdl-12092835
ABSTRACT
An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol the calibration curve of pyridoxal showed high linearity (r=0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation of pyridoxal 5'-phosphate by column chromatography.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Piridoxal
/
Espectrometría de Fluorescencia
/
Oxidorreductasas de Alcohol
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Japón