Regulation of the mouse Nas1 promoter by vitamin D and thyroid hormone.
Pflugers Arch
; 444(3): 353-9, 2002 Jun.
Article
en En
| MEDLINE
| ID: mdl-12111243
ABSTRACT
The renal sodium-sulfate cotransporter, NaS(i)-1, a protein implicated to control serum sulfate levels, has been shown to be regulated in vivo by 1,25-dihydroxyvitamin D(3) (1,25-(OH)(2)D(3)) and tri-iodothyronine (T(3)). Recently, we cloned the mouse NaS(i)-1 gene ( Nas1) and in the present study identified a 1,25-(OH)(2)D(3)- and T(3)-responsive element located within the Nas1 promoter. Mutational analysis of the Nas1 promoter resulted in identification of a direct repeat 6-type vitamin-D-responsive element (DR6 VDRE) at -525 to -508 and an imperfect inverted repeat 0-type T(3)-responsive element (IR0 T(3)RE) at -436 to -425 which conferred 1,25-(OH)(2)D(3) and T(3) responsiveness, respectively. In summary, we have identified responsive elements that mediate the enhanced transcription of Nas1 by 1,25-(OH)(2)D(3) and T(3), and these mechanisms may provide important clues to the physiological control of sulfate homeostasis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Triyodotironina
/
Calcitriol
/
Agonistas de los Canales de Calcio
/
Regiones Promotoras Genéticas
/
Proteínas de Transporte de Catión
/
Simportadores
Límite:
Animals
Idioma:
En
Revista:
Pflugers Arch
Año:
2002
Tipo del documento:
Article
País de afiliación:
Australia