A PCR test for detecting Escherichia coli O157:H7 based on the identification of the small inserted locus (SILO157).
J Appl Microbiol
; 93(2): 250-60, 2002.
Article
en En
| MEDLINE
| ID: mdl-12147073
ABSTRACT
AIMS:
This paper provides identification of a DNA sequence derived from Shiga toxin-producing Escherichia coli (STEC) O157H7 and information on its utilization for detecting STEC O157 by PCR. METHODS ANDRESULTS:
Random Amplified Polymorphic DNA and DNA library were used to identify in STEC O157H7 (strain EDL 933) a 2634-bp Small Inserted Locus, designated SILO157. Analysis of 211 bacterial strains showed that the PCR assays amplifying the SILO157 region could be used to detect STEC O157 with a good specificity.CONCLUSIONS:
Characterization of a novel locus in STEC O157 is attractive since the serotype O157H7 of STEC is still by far the most important serotype associated with more serious diseases. This island encodes putative proteins and especially one that is predicted to be an outer membrane protein designated IHP1. SIGNIFICANCE AND IMPACT OF THE STUDY Further investigations could now be developed to appreciate the role of the SILO157 in pathogenicity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Elementos Transponibles de ADN
/
Técnica del ADN Polimorfo Amplificado Aleatorio
/
Escherichia coli O157
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia