Increased spore production by Glomus intraradices in the split-plate monoxenic culture system by repeated harvest, gel replacement, and resupply of glucose to the mycorrhiza.
Mycorrhiza
; 12(4): 163-7, 2002 Aug.
Article
en En
| MEDLINE
| ID: mdl-12189469
ABSTRACT
Monoxenic culture of Glomus intraradices Schenck and Smith with Ri T-DNA transformed roots in two-compartment Petri dishes is a very useful technique for physiological studies and the production of clean fungal tissues. Experiments were conducted to increase the efficiency of this method for the production of arbuscular mycorrhizal fungus spores. Approximately 20,000 spores could be harvested every 2 months from the distal (fungus only) compartment of a 9-cm-diameter divided Petri dish. The method requires replacement of the gelled media in the distal compartment and resupply of 200 mg glucose to the proximal (root) compartment coincident with harvest of spores. These modifications resulted in an approximate threefold increase in spore production per unit time over the standard split-plate culture technique.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Esporas Fúngicas
/
Micorrizas
/
Hongos
/
Glucosa
Idioma:
En
Revista:
Mycorrhiza
Asunto de la revista:
BIOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos