Ectodomain shedding of furin: kinetics and role of the cysteine-rich region.
FEBS Lett
; 527(1-3): 309-14, 2002 Sep 11.
Article
en En
| MEDLINE
| ID: mdl-12220680
ABSTRACT
Furin, a member of the subtilisin-like pro-protein convertase family, is a type I membrane protein that undergoes ectodomain shedding. Metabolic labeling of cells stably expressing furin demonstrated that the shed form of furin is detected after 30 min. Moreover, sequence analysis revealed that specific residues of the cysteine-rich region of furin aligned with those of tumor necrosis factor receptor, which is also shed. Introduction within furin's cysteine-rich region of mutations that impair TNFR1 shedding also abolished furin shedding. Our results show that shedding of furin occurs rapidly and further suggest that specific cysteine residues may impart a conformation to the enzyme, thereby affecting its susceptibility to proteolysis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Subtilisinas
/
Cisteína
Límite:
Humans
Idioma:
En
Revista:
FEBS Lett
Año:
2002
Tipo del documento:
Article
País de afiliación:
Canadá