The Fusion Expression of HBV preS Epitopes and the Core Antigen.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 28(4): 380-388, 1996.
Article
en En
| MEDLINE
| ID: mdl-12232630
ABSTRACT
The DNA fragments encoding the preS epitopes of HBV surface antigen were fused to the HBc gene and expressed in E. coli under the control of the tac promoter. The products were analyzed by ELISA and Western blotting, which confirmed that the hybrid proteins were expressed as expected. Analysis by electron microscopy and CsCl density gradient ultracentrifugation revealed that all fusion proteins were able to form particles, only with a slightly lower density than the native multimeric HBc. Partially purified fusion particles were then used as immunogen to Balb/c mice and high titer antibody against the preS1(21-47) epitope was observed, which demonstrated that the immunogenictiy of preS1 (21-47) could be greatly improved when fused in the el loop in HBc protein.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
Año:
1996
Tipo del documento:
Article
País de afiliación:
China