Your browser doesn't support javascript.
loading
Molecular forms of NGF in human and rat neuropathic tissues: decreased NGF precursor-like immunoreactivity in human diabetic skin.
Yiangou, Yiangos; Facer, Paul; Sinicropi, Dominick V; Boucher, Tim J; Bennett, David L H; McMahon, Stephen B; Anand, Praveen.
Afiliación
  • Yiangou Y; Peripheral Neuropathy Unit, Imperial College School of Medicine, Hammersmith Hospital, London, UK.
J Peripher Nerv Syst ; 7(3): 190-7, 2002 Sep.
Article en En | MEDLINE | ID: mdl-12365567
Precursors of NGF have been shown to be the predominant forms of this neurotrophin in human brain and peripheral tissues, and proNGF has been shown recently to preferentially bind p75NTR with high affinity. In our studies of human and rat skin and nerve extracts, a 53 kDa band was detected by Western blot using antibodies against rhNGF or prepro-NGF (-91 to -60), that could correspond to a previously described modified prepro-NGF-like molecule. The relative optical intensity of the 53-kDa bands was markedly reduced in skin extracts from patients with subclinical diabetic neuropathy (diabetic: 1.5, 1.0-8.0, n = 6; controls: 52.0, 23.0-85.0, n = 6, p = 0.0022) but was increased in extracts of inflamed colon from patients with Crohn's disease (inflamed: median 12.0, range 0.1-12.0, n = 11: controls: 0.1, 0.1-3.0, n = 8, p = 0.0055). Antibodies to both rhNGF and prepro-NGF immunostained basal keratinocytes in tissue sections of normal human and rat skin showed accumulation of immunoreactivity in nerve fibers distal to sciatic nerve ligation in rats. Prepro-NGF antibody immunostained rat large/medium sensory neurons, whereas only small sensory neurons were stained with antibodies to mature rhNGF, suggesting that prepro-NGF may be preferentially taken up and transported by p75NTR. The different molecular forms derived from prepro-NGF are likely to be of importance in sensory mechanisms, and deserve further investigation.
Asunto(s)
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Precursores de Proteínas / Piel / Regulación hacia Abajo / Factor de Crecimiento Nervioso / Neuropatías Diabéticas Límite: Animals / Humans / Male Idioma: En Revista: J Peripher Nerv Syst Asunto de la revista: NEUROLOGIA Año: 2002 Tipo del documento: Article Pais de publicación: Estados Unidos
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Precursores de Proteínas / Piel / Regulación hacia Abajo / Factor de Crecimiento Nervioso / Neuropatías Diabéticas Límite: Animals / Humans / Male Idioma: En Revista: J Peripher Nerv Syst Asunto de la revista: NEUROLOGIA Año: 2002 Tipo del documento: Article Pais de publicación: Estados Unidos