Lung transplantation by continuous perfusion in an experimental auto-transplant animal model.
Thorac Cardiovasc Surg
; 50(5): 301-5, 2002 Oct.
Article
en En
| MEDLINE
| ID: mdl-12375188
ABSTRACT
OBJECTIVE:
The aim of this study was to evaluate the preservation of the lung using the cold flushing technique in association with continuous perfusion of the organ during static hypothermic storage.METHODS:
In the first phase, the hearts and lungs of 5 New Zealand rabbits were removed three hours after establishing brain death. The left lungs were each conserved in 200 ml of low-potassium UW solution at 10 degrees C for 3 hours of cold ischemia (control group I). The right lungs were also placed in cold storage but were perfused continuously for three hours with low-potassium UW solution at 10 degrees C (group II). In the second phase, ten rabbits underwent a right lung auto-transplant. Lungs were conserved using two techniques. Histoenzymatic and pathological tests were performed lung function was evaluated.RESULTS:
In the first phase the histopathological examination carried out at the end of storage revealed fewer ischemic alterations in the second group compared to the first. In the second phase a significant hypoxia was observed in group I when both lungs and the right lung only were perfused. The histopathological examination revealed ischaemia/reperfusion lesions in both groups though mainly in group I and a good level of ATPase activity in group II though these results were not significant.CONCLUSIONS:
Cold flushing of the pulmonary artery and continuous perfusion during static hypothermic storage appears to guarantee a better partial arterial pressure of oxygen in this model of auto-transplant compared to the classical cold storage method.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Preservación de Órganos
/
Trasplante de Pulmón
/
Soluciones Preservantes de Órganos
Límite:
Animals
Idioma:
En
Revista:
Thorac Cardiovasc Surg
Año:
2002
Tipo del documento:
Article
País de afiliación:
Italia