Evaluation of human hepatocyte incubation as a new tool for metabolism study of androstenedione and norandrostenedione in a doping control perspective.
J Chromatogr B Analyt Technol Biomed Life Sci
; 780(1): 145-53, 2002 Nov 15.
Article
en En
| MEDLINE
| ID: mdl-12383490
ABSTRACT
Human hepatocyte incubations were used to study the metabolism of precursors of testosterone and nortestosterone and to evaluate qualitatively the correlation between in vitro and published in vivo urinary metabolic profiles. Both phase I and phase II biotransformations were observed in vitro oxidoreduction at C-3 and C-17, reduction at C-4,5, hydroxylation at C-6 beta and C-16, glucuronidation and sulfation. All major metabolites detected in urine following oral administration of androstenedione and norandrostenedione were present in human hepatocyte incubations. The good correlation between in vitro and in vivo metabolic profiles indicates that hepatocyte incubations can be a useful tool to identify and characterize metabolites that could be potential urinary markers for detection of steroid abuse by athletes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hepatocitos
/
Doping en los Deportes
/
Androstenodiona
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Canadá