Assignment of backbone 1H, 13C, and 15N resonances of the SH2 domain of human Grb14.

ABSTRACT

The (1)H, (13)C, and (15)N backbone resonance assignments have been made for the Src homology 2 (SH2) domain of the human molecular adapter protein Grb14. The assignments, along with the majority of the non-aromatic side-chain (1)H and (13)C resonances are reported. The SH2 domain has been complexed with a phosphotyrosine-containing peptide (pY766) corresponding to the putative binding site in the fibroblast growth factor receptor (FGFR1). Chemical shift changes upon binding are also reported.