Block in development at the pre-B-II to immature B cell stage in mice without Ig kappa and Ig lambda light chain.
J Immunol
; 170(3): 1354-61, 2003 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-12538695
ABSTRACT
Silencing individual C (constant region) lambda genes in a kappa(-/-) background reduces mature B cell levels, and L chain-deficient (lambda(-/-)kappa(-/-)) mice attain a complete block in B cell development at the stage when L chain rearrangement, resulting in surface IgM expression, should be completed. L chain deficiency prevents B cell receptor association, and L chain function cannot be substituted (e.g., by surrogate L chain). Nevertheless, precursor cell levels, controlled by developmental progression and checkpoint apoptosis, are maintained, and B cell development in the bone marrow is fully retained up to the immature stage. L chain deficiency allows H chain retention in the cytoplasm, but prevents H chain release from the cell, and as a result secondary lymphoid organs are B cell depleted while T cell levels remain normal.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Linfocitos B
/
Cadenas kappa de Inmunoglobulina
/
Cadenas lambda de Inmunoglobulina
Límite:
Animals
Idioma:
En
Revista:
J Immunol
Año:
2003
Tipo del documento:
Article
País de afiliación:
Reino Unido