Selective cholesteryl ester uptake from high density lipoprotein by human first trimester and term villous trophoblast cells.
Placenta
; 24(2-3): 131-43, 2003.
Article
en En
| MEDLINE
| ID: mdl-12566239
ABSTRACT
As villous trophoblast does represent the contact zone between foetal and maternal tissues, the present in vitro study was aimed at investigating cholesterol supply from human high density lipoprotein subclass 3 (HDL(3)) to trophoblast cells isolated from human first trimester and term placenta. Binding of (125)I-HDL(3) was specific and saturable with similar K(d)-values for first trimester (54 microg HDL(3)-protein/ml) and term villous trophoblast cells (29 microg HDL(3)-protein/ml). The cell-association of (125)I-HDL(3) was 3-fold higher for term trophoblast cells while the specific cell-association of [(3)H]cholesterol ester(CE)-labelled HDL(3) was higher for first trimester trophoblast preparations. As a consequence, first trimester trophoblast cells have a pronounced capacity for selective CE-uptake from HDL(3). Competition experiments with native and oxidized low-density lipoprotein as well as cAMP-mediated stimulation of cell-association of [(3)H]CE-HDL(3) in both trophoblast preparations suggested the scavenger receptor class B, type I (SR-BI) as a likely receptor mediating this pathway. SR-BI m RNA could be identified by RT-PCR and Northern blot experiments in both trophoblast preparations. Western blot analysis and immunocytochemistry revealed high expression of SR-BI in first trimester trophoblast. A polyclonal antiserum raised against murine SR-BI significantly decreased cell-association of [(3)H]CE-HDL(3) in trophoblast cells. We conclude that human first trimester and term trophoblast cells express SR-BI which could serve as an efficient route for supplying cholesterol esters from maternal lipoproteins to foetal tissues.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trofoblastos
/
Receptores Inmunológicos
/
Ésteres del Colesterol
/
Vellosidades Coriónicas
/
Receptores de Lipoproteína
/
Proteínas de la Membrana
Límite:
Adult
/
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Placenta
Año:
2003
Tipo del documento:
Article
País de afiliación:
Austria