Role of reactive oxygen species in organophosphate insecticide phosalone toxicity in erythrocytes in vitro.
Toxicol In Vitro
; 17(2): 153-7, 2003 Apr.
Article
en En
| MEDLINE
| ID: mdl-12650668
ABSTRACT
Reactive oxygen species (ROS) caused by organophosphates may be involved in the toxicity of various pesticides. Therefore, in this study we aimed to investigate how an organophosphate insecticide, phosalone, affects lipid peroxidation (LPO) and the antioxidant defence system in vitro. For this purpose, the effects of various doses of phosalone on LPO and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) in erythrocytes were studied. Each phosalone dose was incubated with a previously prepared erythrocyte sample at +4 degrees C for 0, 60 and 180 min. After incubation, the levels of malondialdehyde (MDA) and the activities of SOD, GSH-Px and CAT were determined. Phosalone caused an increase in MDA formation and a decrease in the activities of SOD, GSH-Px and CAT. However, these effects were seen only at extremely high concentrations of phosalone and these concentrations were in the lethal range. Therefore, we suggest that ROS may not involve in the toxic effects of the pesticidal use of phosalone in low concentrations.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Organotiofosforados
/
Inhibidores de la Colinesterasa
/
Especies Reactivas de Oxígeno
/
Eritrocitos
Límite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Toxicol In Vitro
Asunto de la revista:
TOXICOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Turquía